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KMID : 0613820060160030447
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Journal of Life Science
2006 Volume.16 No. 3 p.447 ~ p.453
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Characterization of Arabidopsis Histidine Kinase 3 and Proteomic Analysis of Its Mutant
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Liang Ying-Shi
Cha Jun-Young
Jung Min-Hee
Lee Kon-Ho
Son Dae-Young
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Abstract
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Histidine kinase plays important roles in signal transduction in plant. We characterized the function of Arabidopsis histidine kinase 3 (AHK3) and analyzed the expression patterns of genes and proteins in its mutant ahk3 by trans-zeatin (t-zeatin). The ahk3 exhibited decreased sensitivity to t-zeatin during callus formation, seedling growth, and leaf senescence. From proteomic analysis of ahk3, eukaryotic translation initiation factor 5A-2, auxin binding glutathione S-transferase, and NDPK1 were identified not to be induced by t-zeatin, when compared to the wild-type. In addition, the expression levels of ARR4 and ARR16 among A-type response regulators (ARRs) markedly decreased in ahk3 by t-zeatin treatment. These results suggest that AHK3 plays an important role in cytokinin signaling and the proteins identified from proteomic analysis and specific ARRs, ARR4 and ARR16 may be directly or indirectly associated in AHK3-mediated cytokinin signaling.
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KEYWORD
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Arabidopsis histidine kinase 3, proteomics, response regulators, t-zeatin
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